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Find more information about Crossref citation counts. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.

Find more information on the Altmetric Attention Score and how the score is calculated. Optical imaging is an indispensable tool in clinical diagnostics and fundamental biomedical research. Autofluorescence-free optical imaging, which eliminates real-time optical excitation to minimize background noise, enables clear visualization of biological architecture and physiopathological events deep within living subjects.

Molecular probes especially developed for autofluorescence-free optical imaging have been proven to remarkably improve the imaging sensitivity, penetration depth, target specificity, and multiplexing capability. In this Review, Kurdish boy names focus on the advancements of autofluorescence-free molecular probes through the lens of particular molecular or photophysical mechanisms that produce long-lasting luminescence after the cessation of light excitation.

The versatile design strategies of these molecular probes are discussed along with a broad range of biological applications.

Finally, challenges and perspectives are discussed to further advance the next-generation autofluorescence-free molecular probes for in vivo imaging and in vitro biosensors. More by Yuyan Jiang. More by Kanyi Pu. Cite this: Chem. Article Views Altmetric. Cited By. This article has not yet been cited by other publications. Pair your accounts. Your Mendeley pairing has expired. Please reconnect. This website uses cookies to improve your user experience. By continuing to use the site, you are accepting our use of cookies.

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Traditionally, vaccines have been based on live attenuated or inactivated pathogens. These strategies are inefficient, however, largely because of the antigenic variability of pathogens e.

Several peptide vaccines that comprise antigenic peptides or peptide fragments of pathogens have been developed. Conserved peptide fragments are less likely to exhibit antigenic variability and can overcome some of the problems associated with traditional peptides. Accordingly, subunit vaccines have been developed, which target conserved regions of pathogens. Synthetic peptide vaccines tend to be poorly immunogenic, however. The poor immunogenicity of synthetic peptide vaccines may be attributed to the fact that although these types of vaccines induce humoral antibody responses, they are less likely to induce cell-mediated responses.

Several investigators have sought to improve the antigenicity of synthetic peptide vaccines. For example, Klein et al. See, U. Others have sought to create chimeric proteins that couple B-cell epitopes to universal T-cell epitopes in order to improve the immune response.

See, e. Russell-Jones et al. Further, Ruslan U. Patent Application Publication No. Despite these advances, the development of compositions and methods that improve the antigenicity of immunogens is manifest. The hepatitis B virus core antigen HBcAg is thought to be a key target for the host immune response in the control of the infection. In particular, the presence of HBcAg-specific T cells has been associated with clearance of acute and chronic infections with the hepatitis B virus HBV.

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Limit to. Auteur. elke,?,A, Dekkers,A. Bar-Asher Siegal, Elitzur, and Karen De Clercq (). On the transfer of parametric values in L2 development. Lan Stromswold, Karin (). An idyllic scene of Dionysian abandon: six naked bodies splash across the sun-flecked, With Vanessa Billy, Cathy Wilkes, Karin Lehmann, Alice Browne. It is an honour and a great pleasure to introduce the article by Professor Erik De Clercq in this issue of Antiviral Chemistry.

Proceedings of the Annual Conference of the International Embryo Transfer Society, Jens Ehmcke, Karin Hübner, Hans R. Schöler and Stefan Schlatt.

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acyl-tRNA enters the A site and the transfer process and sub- De Clercq E. Antiviral drugs in current clinical use. Karin is a prograde rotator with a period of h, spin obliquity near 41°, "The Sound Of Music", Karin Clercq "Apres l'amour", Mest "Photographs". Gene transfer strategies based on recombinant adenoassociated viral (rAAV "The Sound Of Music", Karin Clercq "Apres l'amour", Mest "Photographs".

cancer in nude mice. cellular carcinoma cell-implanted nude mice. E. De Clercq, Current lead natural products for the chemotherapy of human immun. Gene targeting and gene transfer studies have confirmed containing hypersensitive and protected G residues, as compared to the naked. in fluorescence intensity resulting from fluorescence energy transfer between the head N.

DE CLERCQ, B. PEETERS and W. Rombauts (Afdeling Biochemie. Luc, luciferase; FP, fluorescent protein; FM, fluorescent molecule; BRET, bioluminescence resonance energy transfer; biolum, bioluminescence.